Journal of Cellular Neuroscience and Oxidative Stress, Vol 2, No 2 (2010)

Ascorbic Acid Mediated Iron Toxicity in Caco-2 Cells:Effects of Different Iron Species

Cem Ekmekcioglu, Bettina Zodl, Susanne Humpeler, Michaela Zeiner, Claudia Gundacker, Ilse Steffan

Abstract


Aim of the presented study was to investigate whether scorbic acid enhances iron uptake and/or toxicity in Caco-2 cells. Cells were incubated with 1.5 mM of different iron species combined with increasing doses of ascorbic acid (1-3 mM). Thereafter viability, membrane damage, lipid peroxidation, and intracellular iron concentrations were measured. No effects on iron-induced damage to cells were found regarding viability.
Lipid peroxidation significantly increased when ascorbic acid was added to cells incubated with FeSO. In 4 contrast lactate dehydrogenase (LDH)-release decreased by addition of ascorbic acid in Caco-2 cells treated with FeCl : nitrilotriacetic acid (NTA) and FeCl : citric acid. Iron uptake was enhanced by ascorbic acid, when 3 3
FeCl : citric acid (1:2) or FeSO were used.

We suggested that, especially dependent on the iron species used, 3 4 a combination of ascorbic acid and iron favours lipid peroxidation in Caco-2 cells. But on the other hand we also found beneficial effects of this type of co-treatment, like an enhanced iron uptake and decreased plasma membrane damage.

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