SDÜ Eğirdir Su Ürünleri Fakültesi Dergisi, Cilt 1, Sayı 1 (2005)

Cryopreservation of Rainbow Trout (Oncorhynchus mykiss) and Mirror Carp (Cyprinus carpio) Sperm with Glucose Based Extender

Yusuf BOZKURT, Selçuk SEÇER, Necmettin TEKİN, Ergun AKÇAY

Özet


In this study, cryopreservation of rainbow trout (Oncorhynchus mykiss) and mirror carp (Cyprinus
carpio) spermatozoa was investigated. Following determination of the spermatological properties, the
pooled ejaculates were diluted with glucose based extender. Diluted sperm was packaged in 0.5 ml straws
and frozen in liquid nitrogen vapour (-196 °C) for 10 minutes and plunged into the liquid nitrogen. The
straws were thawed in a water bath at 30 ºC for 30 seconds. In rainbow trout, the best post-thaw motility
(%), post-thaw movement duration (s) and post-thaw fertilization results (%) were obtained as 57 %, 96 s.
and 68.5 % while the same properties were determined as 64 %, 218 s. and 35 % in mirror carp
respectively.

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